tag:blogger.com,1999:blog-3590966175110441391.post945015656774898427..comments2023-06-26T03:08:40.549-07:00Comments on Tomorrow's Table: Freedom from Hunger- science can lend a helping handPamela Ronaldhttp://www.blogger.com/profile/08905736049638342587noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-3590966175110441391.post-40727702769361864762008-07-12T17:22:00.000-07:002008-07-12T17:22:00.000-07:00Pam, Thanks for the explanation. It is a wonderfu...Pam, <BR/><BR/>Thanks for the explanation. It is a wonderful accomplishment to get this trait into more varieties.<BR/><BR/>- DougAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-3590966175110441391.post-34156608160803019372008-07-11T14:34:00.000-07:002008-07-11T14:34:00.000-07:00hi DougFor the transgenic approach, we initially u...hi Doug<BR/><BR/>For the transgenic approach, we initially use the genomic clone (the native promoter and terminator) and put the construct into the genetic background Liao Gang (a japonica subspecies). We did not see good tolerance so instead we tried to overexpress the cDNA using the Maize ubiquitin promoter. It worked! These plants gave yielded very good submergence tolerance in the japonica variety.<BR/><BR/>The new variety that is now being planted in farmers fields is in the variety Swarna preferred by farmers in the region.Pamela Ronaldhttps://www.blogger.com/profile/08905736049638342587noreply@blogger.comtag:blogger.com,1999:blog-3590966175110441391.post-40637494693407260502008-07-11T12:23:00.000-07:002008-07-11T12:23:00.000-07:00Pam, Good story! Did you ever figure out why the ...Pam, <BR/><BR/>Good story! <BR/><BR/>Did you ever figure out why the transgenic approach for putting submergence tolerance genes into sensitive rice varieties did not work initially (did you finally get it to work)?Anonymousnoreply@blogger.com